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Evidence Guide: AHCSDT408A - Perform an electrophoresis test on a seed sample

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

AHCSDT408A - Perform an electrophoresis test on a seed sample

What evidence can you provide to prove your understanding of each of the following citeria?

Prepare seed extraction and gel

  1. Working sample is prepared from submitted sample.
  2. Seed to be tested is identified and records completed.
  3. Single seeds are cut or crushed and transferred to centrifuge tubes.
  4. Extraction solution is added and mixed with crushed seeds and allowed to stand for prescribed period.
  5. Tubes are centrifuged and supernatants are collected and stored.
  6. Gel mixture is prepared and chemicals and used to achieve required level of polymerisation.
Working sample is prepared from submitted sample.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Seed to be tested is identified and records completed.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Single seeds are cut or crushed and transferred to centrifuge tubes.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Extraction solution is added and mixed with crushed seeds and allowed to stand for prescribed period.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Tubes are centrifuged and supernatants are collected and stored.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Gel mixture is prepared and chemicals and used to achieve required level of polymerisation.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Carry out electrophoresis

  1. Buffer solution is placed in tanks.
  2. Samples are loaded into wells and gel is placed in tanks.
  3. Electrophoresis is carried out at the prescribed voltage and duration.
  4. Temperature is maintained as required throughout the process
Buffer solution is placed in tanks.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Samples are loaded into wells and gel is placed in tanks.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Electrophoresis is carried out at the prescribed voltage and duration.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Temperature is maintained as required throughout the process

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Fix and stain sample

  1. Gel is removed from tank and treated with chemicals as prescribed.
  2. Gel is washed and treated to enhance stain.
  3. Stain is examined and/or photographed.
Gel is removed from tank and treated with chemicals as prescribed.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Gel is washed and treated to enhance stain.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Stain is examined and/or photographed.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Evaluate stain

  1. Banding pattern is compared with that of authentic variety or that of a known control sample.
  2. Records and reports are provided in accordance with enterprise procedures.
  3. Determine if obvious procedure or equipment problems have led to atypical data or results..
Banding pattern is compared with that of authentic variety or that of a known control sample.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Records and reports are provided in accordance with enterprise procedures.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Determine if obvious procedure or equipment problems have led to atypical data or results..

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain a safe work environment

  1. Established safe work practices and personal protective equipment are used to ensure personal safety and that of other laboratory personnel.
  2. The generation of wastes and environmental impacts are minimised.
  3. The safe collection of laboratory and hazardous waste for subsequent disposal is ensured.
  4. Equipment and reagents are cared for and stored as required
Established safe work practices and personal protective equipment are used to ensure personal safety and that of other laboratory personnel.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

The generation of wastes and environmental impacts are minimised.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

The safe collection of laboratory and hazardous waste for subsequent disposal is ensured.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Equipment and reagents are cared for and stored as required

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain laboratory records

  1. Approved data is entered into laboratory information management system.
  2. Confidentiality and security of enterprise information and laboratory data is maintained.
Approved data is entered into laboratory information management system.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Confidentiality and security of enterprise information and laboratory data is maintained.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Evidence Guide

The evidence guide provides advice on assessment and must be read in conjunction with the performance criteria, required skills and knowledge, range statement and the Assessment Guidelines for the Training Package.

Overview of assessment

Critical aspects for assessment and evidence required to demonstrate competency in this Unit

The evidence required to demonstrate competency in this Unit must be relevant to workplace operations and satisfy holistically all of the requirements of the performance criteria and required skills and knowledge and include achievement of the following:

extract seed and prepare gel

centrifuging seed and gel mixes

interpreting stain bands and patterns.

Context of and specific resources for assessment

Competency requires the application of work practices under work conditions. Selection and use of resources for some worksites may differ due to the regional or enterprise circumstances.

Method of assessment

This Unit should be assessed together with core Units and other Units of Competency relevant to the function or work role.

Guidance information for assessment

To ensure consistency of performance, competency should be demonstrated on more than one occasion over a period of time in order to cover a variety of circumstances, cases and responsibilities, and where possible, over a number of assessment activities.

Required Skills and Knowledge

Required skills include:

Ability to:

analyse and report results

evaluation stain banding

fix and stain seed samples

maintain security, integrity, traceability of samples, sub-samples, test data, results and documentation

prepare seed extraction and gel

record and communicate results in accordance with enterprise procedures

select test methods and procedures

use a centrifuge.

Required knowledge includes:

Knowledge of:

seed biology

the role of the International Seed Testing Association (ISTA) and the ISTA Rules for Seed Testing

principles of electrophoresis

range of electrophoresis testing procedures

materials and apparatus used in electrophoresis testing

enterprise standard operating procedures (SOPs)

relevant health, safety and environment requirements

using stain patterns and "fingerpints" to determine seed variety.

Range Statement

The range statement relates to the Unit of Competency as a whole. It allows for different work environments and situations that may affect performance. Bold italicised wording, if used in the performance criteria, is detailed below. Essential operating conditions that may be present with training and assessment (depending on the work situation, needs of the candidate, accessibility of the item, and local industry and regional contexts) may also be included.

Seed may include:

bare seed; treated seed; and coated seed that has been prepared as required.